问题：
[未知题]TDM工作中最常采用的标本为______，现一般均测定其包括_________和_________两部分的总药物浓度，此时可用_________法去蛋白；若仅需测定与药物作用关系更密切部分的药物浓度，应选用_________、_________、_________等方法去蛋白。
参考答案：
血清；游离的；与蛋白结合的；沉淀离心；超滤；层析；超速离心
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